FastQCFastQC Report
Thu 12 May 2022
BS16963A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBS16963A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20311720
Sequences flagged as poor quality0
Sequence length79
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT2489261.2255289064638544TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG1330770.6551734663534157TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT1172660.5773317079991257TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTG225880.11120673187696561TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)