May 25 06:17:45 ..... Started STAR run May 25 06:17:45 ..... Loading genome May 25 06:17:55 ..... Started mapping May 25 06:27:03 ..... Finished successfully [E::hts_open_format] Failed to open file SRR6658278.sorted.bam.tmp.0000.bam samtools sort: failed to create temporary file "SRR6658278.sorted.bam.tmp.0000.bam": File exists [W::bam_hdr_read] EOF marker is absent. The input is probably truncated [E::bgzf_read] Read block operation failed with error 4 after 0 of 4 bytes samtools index: failed to create index for "SRR6658278.sorted.bam": No such file or directory ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.6.3 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P SRR6658278.sorted.bam || || || || Output file : SRR6658278 || || Summary : SRR6658278.summary || || Annotation : genes.gtf (GTF) || || Dir for temp files : /gpfs/data/yanailab/public/linda_share/for_d ... || || || || Threads : 1 || || Level : meta-feature level || || Paired-end : yes || || Multimapping reads : not counted || || Multi-overlapping reads : not counted || || Min overlapping bases : 1 || || || || Chimeric reads : counted || || Both ends mapped : required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file genes.gtf ... || || Features : 325154 || || Meta-features : 24421 || || Chromosomes/contigs : 36 || || || || Process BAM file SRR6658278.sorted.bam... || || Paired-end reads are included. || || Assign alignments (paired-end) to features... || || || || WARNING: reads from the same pair were found not adjacent to each || || other in the input (due to read sorting by location or || || reporting of multi-mapping read pairs). || || || || Pairing up the read pairs. || || || || Total alignments : 22376375 || || Successfully assigned alignments : 17426376 (77.9%) || || Running time : 1.29 minutes || || || || || || Summary of counting results can be found in file "/gpfs/data/yanailab/pub || || lic/linda_share/for_debbie/GSE109972/featurecounts/SRR6658278.summary" || || || \\===================== http://subread.sourceforge.net/ ======================//